Application

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Integrin 1 in COS7, HUVEC, and C6 cell lysates.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Integrin 1 in HeLa, A431, HUVEC, and NIH 3T3 cells.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Integrin 1 in human uterus and human prostate cancer tissue sections.Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.

Anti-Integrin beta1, clone 1A27 ZooMAb, Cat. No. ZRB1230, is a recombinant Rabbit monoclonal antibody that specifically targets Integrin beta1 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

KLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal, cytoplasmic domain of human Integrin beta1, isoform 1.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

30 µg/mL after reconstitution at 25 µL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 1A27 is a ZooMAb rabbit recombinant monoclonal antibody that detects Integrin beta1. It targets an epitope within 23 amino acids from the C-terminal, cytoplasmic domain.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

Integrin beta1 (UniProt: P05556; also known as Fibronectin receptor subunit beta, Glycoprotein IIa, GPIIA, VLA-4 subunit beta, CD29) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12) gene (Gene ID: 3688) in human. Integrins are heterodimeric cell surface receptors composed of a variable alpha subunit and a conserved beta subunit. The exact combination of various alpha and beta subunits dictates the binding specificity of integrins to different ECM components. Integrins are not constitutively active. Activation of an integrin from low ligand-binding affinity state to high ligand-binding affinity state requires conformational change that can originate from either from their cytoplasmic or extracellular interactions. Integrin beta-1 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-20), which is subsequently cleaved off to produce the mature form. The mature form contains an extracellular domain (aa 21-728), a transmembrane domain (aa 729-751), and a cytoplasmic domain (aa 752-798). Integrin beta-1 heterodimerizes with either alpha-1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 or alpha-V to recognize different ligands. For example, alpha-1/beta-1 can recognize the proline hydroxylated sequence in collagen and alpha-2/beta-1 can serve as receptor for fibronectin. It is shown that beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Five different isoforms of integrin beta-1 have been described that are produced by alternative splicing. Isoform 1 is shown to be widely expressed while other isoforms are generally co-expressed with a more restricted distribution. Also, isoform 2 is reported to Interfere with isoform 1 resulting in a dominant negative effect on cell adhesion and migration. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.